利用TMT标记结合2D LC-MS/MS技术分析不同时期水牛睾丸曲精细管差异蛋白质组

doi:10.11843/j.issn.0366-6964.2014.08.009

Abstract

Abstract:

To investigate expression of proteins from buffalo testicular seminiferous tubules at different developmental stages (nepionic and senescence).Tandem mass tag (TMT) coupled to two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS).Identification was based on tandem mass tag (TMT)-coupled to 2D liquid chromatography tandem mass spectrometry (2D LC-MS/MS) using LC-ESI LTQ-Orbitrap Elite MS/MS equipment.Detected proteins were processed by bioinformatics analysis using the SEQUEST search engine.Eighty-nine different proteins were obtained with a more than two-fold change between developmental stages.Compared with the nepionic stage，51 proteins were up-regulated and 38 proteins were down-regulated.TMT-coupled 2D LC-MS/MS was an appropriate method for isolating and identifying protein groups of buffalo testicular seminiferous tubules at different developmental stages.The results provided an experimental basis for exploring changes in protein expression during spermatogenesis，and found biological markers of spermatogenesis，possibly Glutathione S-transferase P，Catalase，Heat shock protein 90-alpha and Heat shock 70 ku protein 1-like.

CLC Number:

S823.8⁺3.2

HUANG Yu-lin，FU Qiang，HUANG De-lun，PAN Hong,HUANG Feng-ling,LIANG Xian-wei，ZHANG Ming. Comparative Analysis of Proteomics at Different Developmental Stages of Buffalo Testicular Seminiferous Tubules by TMT-Coupled 2D LC-MS/MS[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1265-1273.

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References

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Comparative Analysis of Proteomics at Different Developmental Stages of Buffalo Testicular Seminiferous Tubules by TMT-Coupled 2D LC-MS/MS

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